FHL1 antibody (AA 233-246)

Catalog No. ABIN185290

This Goat Polyclonal antibody specifically detects FHL1 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for FHL1 antibody (AA 233-246) (ABIN185290)

Target: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FHL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-FHL1 Antibody

Binding Specificity: AA 233-246

Purpose: SLIMMER / FHL1 isoform B

Sequence: CTVSRVSHPV SKARK

Specificity: This antibody is specific for the FHL-1 isoform B (SLIMMER) and is expected to recognise an epitope corresponding to aa 233-246 of human SLIMMER protein.

Cross-Reactivity: Dog, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CTVSRVSHPVSKARK, from the internal region of the protein sequence according to AAC72886.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 30 kDa band observed in Human Heart lysates is consistent with literature (PMID:10480922, calculated MW of 36.3 kDa according to AAC72886). Recommended concentration: 0.01-0.1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for FHL1

Target: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Alternative Name: FHL1

Background: FHL1, KYO-T, SLIM1, bA535K18.1, four and a half LIM domains 1, Four-and-a-half LIM domains 1, bA535K18.1 (LIM protein SLIMMER), RP11-535K18.1, FHL1A, FHL1B, FLH1A, KYOT, MGC111107, SLIMMER, XMPMA, LIM protein SLIMMER, four-and-a-half Lin11, Isl-1 and Mec-

Gene ID: 2273