RIF1 antibody (AA 2076-2089)

Catalog No. ABIN185304

This anti-RIF1 antibody is a Goat Polyclonal antibody detecting RIF1 in ELISA and IHC. Suitable for Human.

Quick Overview for RIF1 antibody (AA 2076-2089) (ABIN185304)

Target: RIF1 (RAP1 Interacting Factor Homolog (RIF1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This RIF1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-RIF1 Antibody

Binding Specificity: AA 2076-2089

Purpose: RIF1 (aa 2076-2089)

Sequence: CEEGIIDANK TETNT

Specificity: This antibody is expected to recognise an epitope corresponding to aa 2076-2089 of human RIF1 protein.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CEEGIIDANKTETNT, from the internal region of the protein sequence according to NP_060621.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Testis and Brain (Cortex). Recommended concentration: 1-2 μg/mL.

Western Blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the predicted size of approx. 247 kDa according to NP_060621. Therefore we cannot recommend an optimal concentration.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RIF1

Target: RIF1 (RAP1 Interacting Factor Homolog (RIF1))

Alternative Name: RIF1

Background: Rif1, telomere-associated protein RIF1 homolog, Rap1 interacting factor 1, RAP1 interacting factor homolog (yeast), DKFZp434D1026, DKFZp781N1478, FLJ12870, OTTHUMP00000162712, RAP1 interacting factor 1

Gene ID: 55183

NCBI Accession: NP_060621

Pathways: Stem Cell Maintenance