IRAK4 antibody (N-Term)

Catalog No. ABIN185338

This Goat Polyclonal antibody specifically detects IRAK4 in WB, ELISA, IHC, FACS and IF. It exhibits reactivity toward Human.

Quick Overview for IRAK4 antibody (N-Term) (ABIN185338)

Target: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IRAK4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-IRAK4 Antibody

Binding Specificity: N-Term

Purpose: IRAK4 (N Terminus)

Sequence: NKPITPSTYV RC

Cross-Reactivity: Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: NKPITPSTYVRC

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Heart and Human Liver. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 50 kDa band observed in lysates of cell line HeLa and approx 55 kDa in lysates of cell line Jurkat (calculated MW of 51.5 kDa according to NP_057207.1). This molecular weight is routinely observed by other sources. Recommended concentra

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and microtubules of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concent

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for IRAK4

Target: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Alternative Name: IRAK4

Background: IRAK4, REN64, NY-REN-64, interleukin-1 receptor-associated kinase 4, IRAK-4 mutated form 1, interleukin-1 receptor associated kinase 4, interleukin-1 receptor associated kinase 4 mutant form 1, IPD1

Gene ID: 51135, 266632

NCBI Accession: NP_057207, NP_001107654

Pathways: NF-kappaB Signaling, TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades