SRD5A1 antibody (N-Term)

Catalog No. ABIN185346

Cited in 1 publication. The Goat Polyclonal anti-SRD5A1 antibody (ABIN185346) specifically detects SRD5A1 in IHC and ELISA. The antibody is reactive with Human samples.

Quick Overview for SRD5A1 antibody (N-Term) (ABIN185346)

Target: SRD5A1 (Steroid-5-alpha-Reductase, alpha Polypeptide 1 (3-Oxo-5 alpha-Steroid delta 4-Dehydrogenase alpha 1) (SRD5A1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SRD5A1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-SRD5A1 Antibody

Binding Specificity: N-Term

Purpose: SRD5A1 / 5-alpha reductase 1

Sequence: ATATGVAEER LLC

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence ATATGVAEERLLC, from the N Terminus of the protein sequence according to NP_001038.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Prostate shows reticulate staining in the cytoplasm of secretory cells in the gland. Recommended concentration: 4-6 μg/mL. Paraffin embedded Human Liver. Recommended concentration: 5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-SRD5A1 antibody (ABIN185346)

Kapp, Sommer, Kiefer, Dölken, Haendler: "5-alpha-reductase type I (SRD5A1) is up-regulated in non-small cell lung cancer but does not impact proliferation, cell cycle distribution or apoptosis." in: Cancer cell international, Vol. 12, Issue 1, pp. 1, (2012) (PubMed).

Target Details for SRD5A1

Target: SRD5A1 (Steroid-5-alpha-Reductase, alpha Polypeptide 1 (3-Oxo-5 alpha-Steroid delta 4-Dehydrogenase alpha 1) (SRD5A1))

Alternative Name: SRD5A1

Background: SRD5A1, steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1), 5-alpha reductase, steroid 5-alpha-reductase type I, 3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1, steroid-5-alpha-reductase 1

Gene ID: 6715

NCBI Accession: NP_001038

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process