PDE5A antibody (C-Term)

Catalog No. ABIN185350

This Goat Polyclonal antibody specifically detects PDE5A in WB, ELISA, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for PDE5A antibody (C-Term) (ABIN185350)

Target: PDE5A (phosphodiesterase 5A, cGMP-Specific (PDE5A))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PDE5A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-PDE5A Antibody

Binding Specificity: C-Term

Purpose: PDE5A

Sequence: LINGESGQAK RN

Specificity: This antibody is expected to recognise reported isoforms NP_001074.2, NP_236914.2 and NP_246273.2.

Cross-Reactivity: Cow, Dog, Human, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-LINGESGQAKRN, from the C Terminus of the protein sequence according to NP_001074.2, NP_246273.2, NP_236914.2, (NP_237223).

Isotype: IgG

Application Details

Application Notes:

Western Blot: In transfected HEK293 transiently expressing PDE5A isoform 2 a band of approx. 95 kDa is observed. This band is not observed in the non-transfected HEK293 (calculated MW of 94.8 kDa according to isoform 2, NP_236914.2). Recommended concentrat

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PDE5A

Target: PDE5A (phosphodiesterase 5A, cGMP-Specific (PDE5A))

Alternative Name: PDE5A

Background: PDE5A, CN5A, PDE5, PDE5A1, CGB-PDE, phosphodiesterase 5A, cGMP-specific, cGMP-binding cGMP-specific 3',5'-cyclic nucleotide phosphodiesterase

Gene ID: 8654

NCBI Accession: NP_001074, NP_246273, NP_236914

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling