Integrin beta 5 antibody (C-Term)

Catalog No. ABIN185353

This Goat Polyclonal antibody specifically detects Integrin beta 5 in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for Integrin beta 5 antibody (C-Term) (ABIN185353)

Target: Integrin beta 5 (ITGB5)

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Integrin beta 5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-Integrin beta 5 Antibody

Binding Specificity: C-Term

Purpose: Integrin beta 5

Sequence: TFNKFNKSYN GTVD

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TFNKFNKSYNGTVD, from the C Terminus of the protein sequence according to NP_002204.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 110 kDa band observed in Human Ovary and Mouse Eye lysates (calculated MW of 88.1 kDa according to NP_002204.2). Recommended concentration: 0.1-0.3 μg/mL.

Customer feedback: observed 110 kDa in Mouse Eye was confirmed and not present

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Integrin beta 5

Target: Integrin beta 5 (ITGB5)

Alternative Name: ITGB5

Background: ITGB5, integrin, beta 5, FLJ26658

Gene ID: 3693, 16419

NCBI Accession: NP_002204

Pathways: Integrin Complex