WASP antibody (Internal Region)

Catalog No. ABIN185368

This Goat Polyclonal antibody specifically detects WASP in ELISA, IHC, FACS and IF. It exhibits reactivity toward Human.

Quick Overview for WASP antibody (Internal Region) (ABIN185368)

Target: WASP (WAS) (Wiskott-Aldrich Syndrome (Eczema-thrombocytopenia) (WAS))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This WASP antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-WASP Antibody

Binding Specificity: Internal Region

Purpose: Wiskott-Aldrich Syndrome / WASP

Sequence: SPADKKRSGK KKI

Specificity: No cross-reactivity expected with N WASP (WASL).

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SPADKKRSGKKKI, from the internal region of the protein sequence according to NP_000368.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HepG2 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended co

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for WASP

Target: WASP (WAS) (Wiskott-Aldrich Syndrome (Eczema-thrombocytopenia) (WAS))

Alternative Name: WAS

Background: WAS, WASP, Wiskott-Aldrich syndrome (eczema-thrombocytopenia), THC, IMD2, thrombocytopenia 1 (X-linked), Wiskott-Aldrich syndrome (eczema-thrombocytopenia) protein, THC1, Wiskott-Aldrich syndrome protein

Gene ID: 7454

NCBI Accession: NP_000368