Neuroserpin antibody (C-Term)

Catalog No. ABIN185373

The Goat Polyclonal anti-Neuroserpin antibody (ABIN185373) specifically detects Neuroserpin in WB, ELISA, IHC and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Neuroserpin antibody (C-Term) (ABIN185373)

Target: Neuroserpin (SERPINI1) (serpin Peptidase Inhibitor, Clade I (neuroserpin), Member 1 (SERPINI1))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Neuroserpin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-Neuroserpin Antibody

Binding Specificity: C-Term

Purpose: Neuroserpin

Sequence: TMNTSGHDFE EL

Specificity: Reported variants represent identical protein (NP_005016.1, NP_001116224.1).

Cross-Reactivity: Cow, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TMNTSGHDFEEL, from the C Terminus of the protein sequence according to NP_005016.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5 μg/mL.

Western Blot: Approx 48 kDa band observed in lysates of cell lines Kelly and K562 and in Human Placenta and Mouse and Rat Brain lysates (calculated MW of 46.4 kDa according to Human NP_005016.1, Mouse NP_033276.1 and Rat NP_446231.1). Recommended concentra

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Neuroserpin

Target: Neuroserpin (SERPINI1) (serpin Peptidase Inhibitor, Clade I (neuroserpin), Member 1 (SERPINI1))

Alternative Name: SERPINI1

Background: SERPINI1, neuroserpin, PI12, serine (or cysteine) proteinase inhibitor, clade I (neuroserpin), member 1, protease inhibitor 12 (neuroserpin), serpin peptidase inhibitor, clade I (neuroserpin), member 1, DKFZp781N13156

Gene ID: 5274

NCBI Accession: NP_005016

Pathways: Regulation of Hormone Metabolic Process