AGRP antibody (C-Term)

Catalog No. ABIN185385

This anti-AGRP antibody is a Goat Polyclonal antibody detecting AGRP in IHC and ELISA. Suitable for Human.

Quick Overview for AGRP antibody (C-Term) (ABIN185385)

Target: AGRP (Agouti Related Protein Homolog (Mouse) (AGRP))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This AGRP antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-AGRP Antibody

Binding Specificity: C-Term

Purpose: AGRP / Agouti-related protein

Sequence: CRKLGTAMNP CSRT

Specificity: This antibody is expected to recognise both reported isoforms (as represented by NP_001129, NP_015531)

Cross-Reactivity: Dog, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CRKLGTAMNPCSRT, from the C Terminus of the protein sequence according to NP_001129.1, NP_015531.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Frozen section of Human Hypothalamus shows staining of a group of neuronal cell bodies in the human infundibular (arcuate) nucleus and their varicose axons. Recommended concentration: 0.05-0.1 μg/mL.

Western Blot: Preliminary experiments gave bands at approx 45 kDa and 24 kDa in Human Brain lysates after 0.1 μg/mL antibody staining. Thiese bands were successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temper

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for AGRP

Target: AGRP (Agouti Related Protein Homolog (Mouse) (AGRP))

Alternative Name: AGRP

Background: AGRP, ART, AGRT, ASIP2, agouti related protein homolog (mouse), agouti (mouse) related protein, Agouti-related transcript, mouse, homolog of, MGC118963, agouti related protein homolog

Gene ID: 181

NCBI Accession: NP_001129, NP_015531

Pathways: Feeding Behaviour, Photoperiodism