GAD65 antibody (Internal Region)

Catalog No. ABIN185388

This anti-GAD65 antibody is a Goat Polyclonal antibody detecting GAD65 in WB, IHC and ELISA. Suitable for Human.

Quick Overview for GAD65 antibody (Internal Region) (ABIN185388)

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GAD65 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-GAD65 Antibody

Binding Specificity: Internal Region

Purpose: GAD2 / GAD65

Sequence: TLEDNEERMS RLSK

Specificity: This antibody is expected to recognise both reported variants.

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-TLEDNEERMSRLSK

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 6-8 μg/mL.

Western Blot: Approx 65 kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 65.4 kDa according to NP_000809.1). Recommended concentration: 0.5-1.5 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GAD65

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Alternative Name: GAD2

Background: GAD2, GAD65, glutamate decarboxylase 2 (pancreatic islets and brain, 65 kDa), Glutamate decarboxylase-2 (pancreas), glutamate decarboxylase 2 (pancreatic islets and brain, 65kD), RP11-420F12.2, MGC161605, MGC161607, Glutamate decarboxylase-2 (pancreas), gl

Gene ID: 2572

NCBI Accession: NP_000809, NP_001127838