SNAP25 antibody (C-Term)

Catalog No. ABIN185392

This Goat Polyclonal antibody specifically detects SNAP25 in WB and ELISA. It exhibits reactivity toward Human, Rat and Mouseand has been mentioned in 1 publication.

Quick Overview for SNAP25 antibody (C-Term) (ABIN185392)

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Reactivity: Human, Rat, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This SNAP25 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-SNAP25 Antibody

Binding Specificity: C-Term

Purpose: SNAP25

Sequence: DEANQRATKM LGSG

Specificity: This antibody is expected to recognise both reported isoforms NP_003072.2 (SNAP25A) and NP_570824.1 (SNAP25B). Reported variants represent identical protein: NP_001309831.1, NP_003072.2 Reported variants represent identical protein: NP_001309834.1, NP_001

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DEANQRATKMLGSG, from the C Terminus of the protein sequence according to NP_003072.2, NP_570824.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 26 kDa band observed in Human Brain (Cerebellum) ,Mouse and Rat Brain lysates (calculated MW of 23 kDa and of 23.3 kDa according to NP_003072.2, NP_570824.1). Recommended concentration: 0.005-0.01 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-SNAP25 antibody (ABIN185392)

Kawakami, Ohmoto, Itoh, Yuasa, Inagaki, Nishimura, Ito, Misaka: "Accumulation of SNAP25 in mouse gustatory and somatosensory cortices in response to food and chemical stimulation." in: Neuroscience, Vol. 218, pp. 326-34, (2012) (PubMed).

Target Details for SNAP25

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Alternative Name: SNAP25

Background: SNAP25, synaptosomal-associated protein, 25 kDa , HGNC:11132, FLJ23079, SNAP, SNAP-25, bA416N4.2, dJ1068F16.2 , synaptosomal-associated protein 25, RIC-4, RIC4, SEC9, resistance to inhibitors of cholinesterase 4 homolog, synaptosomal-associated protein 25

Gene ID: 6616, 20614, 25012

NCBI Accession: NP_003072, NP_570824

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport