LRP5 antibody (Internal Region)

Catalog No. ABIN185410

Product Details anti-LRP5 Antibody

Target: LRP5 (Low Density Lipoprotein Receptor-Related Protein 5 (LRP5))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This LRP5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: LRP5

Sequence: ERVEKTTGDK RT

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-ERVEKTTGDKRT, from the internal region of the protein sequence according to NP_002326.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Liver shows weak cytoplasm staining in some hepatocytes. Recommended concentration: 2-4 μg/mL.

Western Blot: Approx 180 kDa band observed in lysates of cell line HepG2 (calculated MW of 179 kDa according to NP_002326.2). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse Liver for this p

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-LRP5 antibody (ABIN185410)

Dong, Li, Li, Ren, Fan, Weng: "MicroRNA-23a-3p inhibitor decreases osteonecrosis incidence in a rat model." in: Molecular medicine reports, Vol. 16, Issue 6, pp. 9331-9336, (2018) (PubMed).

Target Details for LRP5

Target: LRP5 (Low Density Lipoprotein Receptor-Related Protein 5 (LRP5))

Alternative Name: LRP5 (LRP5 Products)

Synonyms: Xlrp5 antibody, bmnd1 antibody, evr1 antibody, evr4 antibody, hbm antibody, lr3 antibody, lrp5-A antibody, lrp7 antibody, oppg antibody, ops antibody, opta1 antibody, vbch2 antibody, LRP5 antibody, LRP6 antibody, BMND1 antibody, EVR1 antibody, EVR4 antibody, HBM antibody, LR3 antibody, LRP7 antibody, OPPG antibody, OPS antibody, OPTA1 antibody, VBCH2 antibody, mKIAA4142 antibody, low-density lipoprotein receptor-related protein 5 antibody, LDL receptor related protein 5 antibody, LDL receptor related protein 5 L homeolog antibody, low density lipoprotein receptor-related protein 5 antibody, LDL receptor related protein 5 like antibody, LOC100147353 antibody, LRP5 antibody, lrp5.L antibody, lrp5 antibody, Lrp5 antibody, LRP5L antibody

Background: LRP5, low density lipoprotein receptor-related protein 5, HGNC:6697, BMND1, HBM, LR3, LRP7, OPPG, OPS, VBCH2, low density lipoprotein receptor-related protein 7, osteoporosis pseudoglioma syndrome, EVR1, EVR4, OPTA1, exudative vitreoretinopathy 1,

Gene ID: 4041

NCBI Accession: NP_002326

Pathways: WNT Signaling, Stem Cell Maintenance, Positive Regulation of fat Cell Differentiation