SYK antibody (Internal Region)

Catalog No. ABIN185423

The Goat Polyclonal anti-SYK antibody has been validated for WB and ELISA. It is suitable to detect SYK in samples from Human.

Quick Overview for SYK antibody (Internal Region) (ABIN185423)

Target: SYK (Spleen tyrosine Kinase (SYK))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SYK antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-SYK Antibody

Binding Specificity: Internal Region

Purpose: Spleen tyrosine kinase / SYK

Sequence: KYLQQNRHVK DKN

Specificity: This antibody is expected to recognise both reported isoforms (NP_003168.2, NP_001128524.1). Reported pairs of variants represent identical proteins (NP_003168.2 and NP_001167638.1, NP_001128524.1 and NP_001167639.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KYLQQNRHVKDKN, from the internal region of the protein sequence according to NP_003168.2, NP_001128524.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 70 kDa band observed in Human Bone marrow, Lymph Node, and Spleen lysates (calculated MW of 72.1 kDa according to NP_003168.2). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SYK

Target: SYK (Spleen tyrosine Kinase (SYK))

Alternative Name: SYK

Background: SYK, spleen tyrosine kinase, HGNC:11491, DKFZp313N1010, FLJ25043, FLJ37489, OTTHUMP00000021628, OTTHUMP00000021629

Gene ID: 6850, 20963, 25155

NCBI Accession: NP_003168, NP_001128524

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Thromboxane A2 Receptor Signaling, BCR Signaling