Neuroligin 1 antibody (Internal Region)

Catalog No. ABIN185446

This Goat Polyclonal antibody specifically detects Neuroligin 1 in ELISA and IF. It exhibits reactivity toward Human.

Quick Overview for Neuroligin 1 antibody (Internal Region) (ABIN185446)

Target: Neuroligin 1 (NLGN1)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Neuroligin 1 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-Neuroligin 1 Antibody

Binding Specificity: Internal Region

Purpose: Neuroligin 1

Sequence: TAKQDDPKQQ

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TAKQDDPKQQ, from the internal region of the protein sequence according to NP_055747.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments gave an approx 150 kDa band in Human Brain lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 92.0 k

Peptide ELISA: antibody detection limit dilution 1:4000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the membranes/ cytoplasm of U251 and U2OS cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Neuroligin 1

Target: Neuroligin 1 (NLGN1)

Alternative Name: NLGN1

Background: NLGN1, KIAA1070, MGC45115, neuroligin 1, OTTHUMP00000211275, OTTHUMP00000211276

Gene ID: 22871

NCBI Accession: NP_055747

Pathways: Synaptic Membrane, Synaptic Vesicle Exocytosis