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Neuroligin 1 antibody (Internal Region)

Verified NLGN1 Reactivity: Human ELISA, IF Host: Goat Polyclonal unconjugated
Catalog No. ABIN185446
  • Target See all Neuroligin 1 (NLGN1) Antibodies
    Neuroligin 1 (NLGN1)
    Binding Specificity
    • 21
    • 16
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 42
    • 36
    • 26
    • 1
    Human
    Host
    • 34
    • 27
    Goat
    Clonality
    • 37
    • 24
    Polyclonal
    Conjugate
    • 23
    • 5
    • 5
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Neuroligin 1 antibody is un-conjugated
    Application
    • 54
    • 26
    • 26
    • 14
    • 13
    • 13
    • 3
    • 1
    • 1
    • 1
    ELISA, Immunofluorescence (IF)
    Purpose
    Neuroligin 1
    Sequence
    TAKQDDPKQQ
    Cross-Reactivity
    Cow, Dog, Human, Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-TAKQDDPKQQ, from the internal region of the protein sequence according to NP_055747.1.
    Isotype
    IgG
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  • Application Notes

    Western Blot: Preliminary experiments gave an approx 150 kDa band in Human Brain lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 92.0 k

    Peptide ELISA: antibody detection limit dilution 1:4000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the membranes/ cytoplasm of U251 and U2OS cells. Recommended concentration: 10μg/ml.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    Neuroligin 1 (NLGN1)
    Alternative Name
    NLGN1 (NLGN1 Products)
    Synonyms
    NLGN1 antibody, Nlgn1 antibody, GB18720 antibody, nlgn1 antibody, NL1 antibody, 6330415N05Rik antibody, BB179718 antibody, mKIAA1070 antibody, neuroligin-1 antibody, neuroligin 1 antibody, NLGN1 antibody, LOC659779 antibody, LOC724358 antibody, nlgn1 antibody, Nlgn1 antibody
    Background
    NLGN1, KIAA1070, MGC45115, neuroligin 1, OTTHUMP00000211275, OTTHUMP00000211276
    Gene ID
    22871
    NCBI Accession
    NP_055747
    Pathways
    Synaptic Membrane, Synaptic Vesicle Exocytosis
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