GGA3 antibody (C-Term)

Catalog No. ABIN185453

The Goat Polyclonal anti-GGA3 antibody is suitable to detect GGA3 in samples from Human. It has been validated for ELISA and IHC.

Quick Overview for GGA3 antibody (C-Term) (ABIN185453)

Target: GGA3 (Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 3 (GGA3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GGA3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-GGA3 Antibody

Binding Specificity: C-Term

Purpose: GGA3

Sequence: EQLSTEVGEV DQ

Specificity: This antibody is expected to recognise both reported isoforms (NP_619525 and NP_054720)

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EQLSTEVGEVDQ, from the C Terminus of the protein sequence according to NP_619525.1, NP_054720.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Preliminary experiments gave an approx 65 kDa band in Mouse and Rat Brain lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GGA3

Target: GGA3 (Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 3 (GGA3))

Alternative Name: GGA3

Background: GGA3, golgi associated, gamma adaptin ear containing, ARF binding protein 3, HGNC:17079, KIAA0154, ADP-ribosylation factor binding protein 3, Golgi-localized, gamma ear-containing, ARF-binding protein 3

Gene ID: 23163

NCBI Accession: NP_619525, NP_054720