Clusterin antibody (C-Term)

Catalog No. ABIN185475

This anti-Clusterin antibody is a Goat Polyclonal antibody detecting Clusterin in WB, IHC, ELISA and IF. Suitable for Human.

Quick Overview for Clusterin antibody (C-Term) (ABIN185475)

Target: Clusterin (CLU)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Clusterin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-Clusterin Antibody

Binding Specificity: C-Term

Purpose: Clusterin / APOJ

Sequence: EKALQEYRKK HREE

Specificity: This antibody is expected to recognise both reported isoforms (NP_001822.2 and NP_976084.1)

Cross-Reactivity: Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EKALQEYRKKHREE, from the C Terminus of the protein sequence according to NP_001822.2, NP_976084.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 35-37 kDa band observed in Human Colon and Prostate lysates (calculated MW of 57.8 kDa according to NP_NP_001822.2 and 52.5 according to NP_976084.1). The observed molecular weight corresponds to earlier findings in literature (PMID: 9

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Clusterin

Target: Clusterin (CLU)

Alternative Name: CLU

Background: CLU, APOJ, clusterin, complement lysis inhibitor, SP-40,40, sulfated glycoprotein 2, testosterone-repressed prostate message 2, apolipoprotein J , HGNC:2095, CLI, MGC24903, SGP-2, SGP2, SP-40, TRPM-2, TRPM2 , apolipoprotein J, clusterin, complement-associ

Gene ID: 1191

NCBI Accession: NP_001822, NP_976084

Pathways: Apoptosis, Negative Regulation of intrinsic apoptotic Signaling