CBX1 antibody (Internal Region)

Catalog No. ABIN185492

The Goat Polyclonal anti-CBX1 antibody has been validated for WB, ELISA and IHC. It is suitable to detect CBX1 in samples from Human.

Quick Overview for CBX1 antibody (Internal Region) (ABIN185492)

Target: CBX1 (Chromobox Homolog 1 (CBX1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CBX1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-CBX1 Antibody

Binding Specificity: Internal Region

Purpose: CBX1 / HP1-Beta

Sequence: QSQKTAHETD KSE

Specificity: Variants (NP_006798.1, NP_001120700.1) encode the same protein.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QSQKTAHETDKSE, from the internal region of the protein sequence according to NP_006798.1, NP_001120700.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Placenta shows strong nuclear staining in trophoblasts. Recommended concentration, 2-3 μg/mL.

Western Blot: Approx. 25 kDa band observed in lysates of A431, HeLa and HepG2 (calculated MW of 25 kDa according to NP_006798.1). Recommended concentration: 0.3-1.0 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CBX1

Target: CBX1 (Chromobox Homolog 1 (CBX1))

Alternative Name: CBX1

Background: CBX1, HP1-BETA, chromobox homolog 1 (HP1 beta homolog Drosophila ), HGNC:1551, CBX, HP1Hs-beta, M31, MOD1, chromobox homolog 1 (Drosophila HP1 beta), heterochromatin protein p25 beta, HP1Hsbeta, p25beta, heterochromatin protein 1-beta, modifier 1 protein

Gene ID: 10951, 12412, 360609

NCBI Accession: NP_006798, NP_001120700