SIRT3 antibody (C-Term)

Catalog No. ABIN185498

The Goat Polyclonal anti-SIRT3 antibody has been validated for WB and ELISA. It is suitable to detect SIRT3 in samples from Human.

Quick Overview for SIRT3 antibody (C-Term) (ABIN185498)

Target: SIRT3 (Sirtuin 3 (SIRT3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SIRT3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-SIRT3 Antibody

Binding Specificity: C-Term

Purpose: SIRT3

Sequence: QRETGKLDGP DK

Specificity: This antibody is expected to recognize both reported isoforms (NP_036371.1 and NP_001017524.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QRETGKLDGPDK, from the C Terminus of the protein sequence according to NP_036371.1, NP_001017524.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 28 kDa band observed in Human Liver lysates (calculated MW of 28.6 kDa according to NP_001017524.1). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SIRT3

Target: SIRT3 (Sirtuin 3 (SIRT3))

Alternative Name: SIRT3

Background: SIRT3, sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae), HGNC:14931, SIR2L3, mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase, silent mating type information regulation 2, S.cerevisiae, homolog 3, sir2-l

Gene ID: 23410

NCBI Accession: NP_036371, NP_001017524