PDPK1 antibody (Internal Region)

Catalog No. ABIN185505

The Goat Polyclonal anti-PDPK1 antibody (ABIN185505) specifically detects PDPK1 in WB and ELISA. The antibody is reactive with Rat samples.

Quick Overview for PDPK1 antibody (Internal Region) (ABIN185505)

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This PDPK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-PDPK1 Antibody

Binding Specificity: Internal Region

Purpose: PDK1

Sequence: DFKDKSAEDA K

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DFKDKSAEDAK, from the internal region of the protein sequence according to NP_002601.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 65-70 kDa band observed in Rat Heart lysates (calculated MW of 49.2 kDa according to NP_002601.1). This molecular weight is routinely observed by other sources. Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:1000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PDPK1

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Alternative Name: PDK1

Background: PDK1, pyruvate dehydrogenase kinase, isoenzyme 1, HGNC:8809, mitochondrial pyruvate dehydrogenase kinase isoenzyme 1, pyruvate dehydrogenase kinase, isoenzyme 1, OTTHUMP00000205076, mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1, pyru

Gene ID: 5163

NCBI Accession: NP_002601

Pathways: PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals