Monoamine Oxidase B antibody (Internal Region)

Catalog No. ABIN185506

This Goat Polyclonal antibody specifically detects Monoamine Oxidase B in WB, ELISA, IF and FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for Monoamine Oxidase B antibody (Internal Region) (ABIN185506)

Target: Monoamine Oxidase B (MAOB)

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Monoamine Oxidase B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-Monoamine Oxidase B Antibody

Binding Specificity: Internal Region

Purpose: MAOB

Sequence: HKARKLARLT KEE

Specificity: This antibody is not expected to cross-react with MAOA.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HKARKLARLTKEE, from the internal region of the protein sequence according to NP_000889.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 60 kDa band observed in Human and Mouse Liver lysates and approx. 55-60 kDa in Human Testis and Breast cancer lysates (calculated MW of 58.8 kDa according to NP_000889.3). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 ho

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentratio

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Monoamine Oxidase B

Target: Monoamine Oxidase B (MAOB)

Alternative Name: MAOB

Background: MAOB, monoamine oxidase B , HGNC:6834, MGC26382, RP1-201D17__B.1, MAO, brain, MAO, platelet, adrenalin oxidase, amine oxidase (flavin-containing), tyramine oxidase

Gene ID: 4129, 109731

NCBI Accession: NP_000889