UCP1 antibody (C-Term)

Catalog No. ABIN185512

This Goat Polyclonal antibody specifically detects UCP1 in WB and ELISA. It exhibits reactivity toward Rat.

Quick Overview for UCP1 antibody (C-Term) (ABIN185512)

Target: UCP1 (Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This UCP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-UCP1 Antibody

Binding Specificity: C-Term

Purpose: UCP1

Sequence: EQLKRELSKS RQ

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EQLKRELSKSRQ, from the C Terminus of the protein sequence according to NP_068605.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 35 kDa band observed in Rat Adipose tissue and Skeletal Muscle lysates (calculated MW of 33.2 kDa according to Rat NP_036814.1). Recommended concentration: 0.5-1 μg/mL. . Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for UCP1

Target: UCP1 (Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))

Alternative Name: UCP1

Background: UCP1, uncoupling protein 1 (mitochondrial, proton carrier), HGNC:12517, SLC25A7, UCP, mitochondrial brown fat uncoupling protein, uncoupling protein 1

Gene ID: 7350

NCBI Accession: NP_068605

Pathways: Proton Transport, Brown Fat Cell Differentiation