NOTCH3 antibody (C-Term)

Catalog No. ABIN185524

This Goat Polyclonal antibody specifically detects NOTCH3 in ELISA, IHC and FACS. It exhibits reactivity toward Human.

Quick Overview for NOTCH3 antibody (C-Term) (ABIN185524)

Target: NOTCH3 (Notch 3 (NOTCH3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NOTCH3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-NOTCH3 Antibody

Binding Specificity: C-Term

Purpose: NOTCH3

Sequence: QLGPQPEVTP KRQ

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QLGPQPEVTPKRQ, from the C Terminus of the protein sequence according to NP_000426.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Hippocampus shows select nuclear staining and weaker staining in different comparments of the neuronal cell bodies. Recommended concentration: 4-6 μg/mL. Paraffin embedded Human Placenta. Recommended concen

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NOTCH3

Target: NOTCH3 (Notch 3 (NOTCH3))

Alternative Name: NOTCH3

Background: NOTCH3, Notch homolog 3 (Drosophila), HGNC:7883, CADASIL, CASIL , Notch (Drosophila) homolog 3, Notch homolog 3

Gene ID: 4854

NCBI Accession: NP_000426

Pathways: Notch Signaling