ROBO1 antibody (Internal Region)

Catalog No. ABIN185537

The Goat Polyclonal anti-ROBO1 antibody has been validated for ELISA, IHC and IF. It is suitable to detect ROBO1 in samples from Human.

Quick Overview for ROBO1 antibody (Internal Region) (ABIN185537)

Target: ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ROBO1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Verified

Product Details anti-ROBO1 Antibody

Binding Specificity: Internal Region

Purpose: ROBO1 / DUTT1 (Internal)

Sequence: GDVDLSNKIN EMK

Specificity: Not expected to cross-react with ROBO2 or ROBO3.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-GDVDLSNKINEMK, from the internal region of the protein sequence according to NP_002932.1, NP_598334.1, NP_001139316.1, NP_001139317.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex) and Kidney. Recommended concentration: 5-6 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the Plasma membrane and Golgi/ER of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ROBO1

Target: ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))

Alternative Name: ROBO1

Background: ROBO1, DUTT1, roundabout, axon guidance receptor, homolog 1 (Drosophila) , HGNC:10249, FLJ21882, SAX3, axon guidance receptor, roundabout 1, MGC131599, MGC133277

Gene ID: 6091, 19876, 58946

NCBI Accession: NP_002932, NP_598334

Pathways: Positive Regulation of Endopeptidase Activity