VPS25 antibody (Internal Region)

Catalog No. ABIN185544

The Goat Polyclonal anti-VPS25 antibody (ABIN185544) specifically detects VPS25 in WB, ELISA and IHC. The antibody is reactive with Human and Rat samples.

Quick Overview for VPS25 antibody (Internal Region) (ABIN185544)

Target: VPS25 (Vacuolar Protein-Sorting-Associated Protein 25 (VPS25))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This VPS25 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-VPS25 Antibody

Binding Specificity: Internal Region

Purpose: VPS25

Sequence: QPNVDTRQKQ

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QPNVDTRQKQ, from the internal region of the protein sequence according to NP_115729.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 6-8 μg/mL.

Western Blot: Approx. 22 kDa band observed in Rat Kidney lysates (calculated MW of 20.7 kDa according to human NP_115729.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for VPS25

Target: VPS25 (Vacuolar Protein-Sorting-Associated Protein 25 (VPS25))

Alternative Name: VPS25

Background: VPS25, vacuolar protein sorting 25 (yeast), HGNC:28122, DERP9, EAP20, MGC10540, vacuolar protein sorting 25, vacuolar protein sorting 25 homolog (S. cerevisiae), FAP20

Gene ID: 84313

NCBI Accession: NP_115729