IFT88 antibody (C-Term)

Catalog No. ABIN185555

The Goat Polyclonal anti-IFT88 antibody has been validated for WB, IF, ELISA and FACS. It is suitable to detect IFT88 in samples from Human and Mouse. There are 2+ publications available.

Quick Overview for IFT88 antibody (C-Term) (ABIN185555)

Target: IFT88 (Intraflagellar Transport 88 Homolog (IFT88))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This IFT88 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-IFT88 Antibody

Binding Specificity: C-Term

Purpose: IFT88 / Polaris

Sequence: KKRIDEDDFA DEE

Specificity: This antibody is expected to recognise many reported isoforms.

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KKRIDEDDFADEE, from the C Terminus of the protein sequence according to NP_783195.2, NP_006522.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 90 kDa band observed in lysates of cell line A549 and in Mouse Testis lysates, and in preliminary testing of Rat Testes lysate (calculated MW of 90.4 kDa according to Hunab NP_001340498.1, and 93.1 kDa according to Mouse NP_033402.2 93.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nucleus and less staining in the cytoplasm of HepG2 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-IFT88 antibody (ABIN185555)

Miyoshi, Kasahara, Miyazaki, Shimizu, Taniguchi, Matsuzaki, Tohyama, Asanuma: "Pericentrin, a centrosomal protein related to microcephalic primordial dwarfism, is required for olfactory cilia assembly in mice." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 23, Issue 10, pp. 3289-97, (2009) (PubMed).

Jiang, Chiou, Wang, Chien, Ho, Tsai, Lin, Tsai, Li: "Essential role of nephrocystin in photoreceptor intraflagellar transport in mouse." in: Human molecular genetics, Vol. 18, Issue 9, pp. 1566-77, (2009) (PubMed).

Target Details for IFT88

Target: IFT88 (Intraflagellar Transport 88 Homolog (IFT88))

Alternative Name: IFT88

Background: IFT88, polaris, TTC10, tetratricopeptide repeat domain 10, HGNC:20606, D13S1056E, DAF19, IFT88, MGC26259, RP11-172H24.2, TG737, hTg737, TPR repeat protein 10, Probe hTg737 (polycystic kidney disease, autosomal recessive), Tg737 protein, intraflagellar tra

Gene ID: 8100, 21821, 305918

NCBI Accession: NP_783195, NP_006522, NP_001340507, NP_001340498

Pathways: Hedgehog Signaling, Sensory Perception of Sound