ARHGEF5 antibody (Internal Region)

Catalog No. ABIN185572

This Goat Polyclonal antibody specifically detects ARHGEF5 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for ARHGEF5 antibody (Internal Region) (ABIN185572)

Target: ARHGEF5 (rho Guanine Nucleotide Exchange Factor (GEF) 5 (ARHGEF5))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ARHGEF5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-ARHGEF5 Antibody

Binding Specificity: Internal Region

Purpose: GEF5

Sequence: DKEIDQNSQQ EE

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DKEIDQNSQQEE, from the internal region of the protein sequence according to NP_005426.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Placenta. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 170 kDa band observed in Human HeLa lysates (calculated MW of 177 kDa according to NP_005426.2). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ARHGEF5

Target: ARHGEF5 (rho Guanine Nucleotide Exchange Factor (GEF) 5 (ARHGEF5))

Alternative Name: ARHGEF5

Background: GEF5, DKFZp686N1969, ARHGEF5, Rho guanine nucleotide exchange factor (GEF) 5, P60, TIM, TIM1, guanine nucleotide regulatory protein TIM, oncogene TIM rho guanine nucleotide exchange factor 5, transforming immortalized mammary oncogene

Gene ID: 7984

NCBI Accession: NP_005426