Iba1 antibody (Internal Region)

Catalog No. ABIN185597

The Goat Polyclonal anti-Iba1 antibody has been validated for IHC, ELISA, IF and FACS. It is suitable to detect Iba1 in samples from Human.

Quick Overview for Iba1 antibody (Internal Region) (ABIN185597)

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Iba1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-Iba1 Antibody

Binding Specificity: Internal Region

Purpose: AIF1 / IBA1 (isoform 3)

Sequence: NKQFLDDPKY SSDED

Specificity: This antibody is expected to recognise isoform 3 (NP_001614.3) only.

Cross-Reactivity: Dog, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NKQFLDDPKYSSDED, from the internal region of the protein sequence according to NP_001614.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Lung and Brain (Cortex). Recommended concentration: 5-6 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and plasma membrane of U937, Caco-2 and U251 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of K562 cells

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Iba1

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Alternative Name: AIF1

Background: AIF1, IBA1, allograft inflammatory factor 1 , DASS-82G15.2, AIF-1, IRT-1, Em:AF129756.17, interferon gamma responsive transcript, ionized calcium-binding adapter molecule

Gene ID: 199, 11629

NCBI Accession: NP_001614

Pathways: Smooth Muscle Cell Migration