PGRMC1 antibody (C-Term)

Catalog No. ABIN185610

This anti-PGRMC1 antibody is a Goat Polyclonal antibody detecting PGRMC1 in WB, IHC, ELISA and IF. Suitable for Human and Mouse. This Primary Antibody has been cited in 1 publication.

Quick Overview for PGRMC1 antibody (C-Term) (ABIN185610)

Target: PGRMC1 (Progesterone Receptor Membrane Component 1 (PGRMC1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This PGRMC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-PGRMC1 Antibody

Binding Specificity: C-Term

Purpose: PGRMC1 / MPR

Sequence: EPKDESARKN D

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EPKDESARKND, from the C Terminus of the protein sequence according to NP_006658.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 5 μg/mL.

Western Blot: Approx 24 kDa band observed in Human Kidney, Liver and Testis lysates, and approx 25 kDa in Mouse Testis lysates (calculated MW of 21.7 kDa according to Human NP_006658.1 and Mouse NP_058063.2). These molecular weights are routinely observed b

Peptide ELISA: antibody detection limit dilution 1:4000.

Comment:

Immunofluorescence: This antibody has been successfully used in IF on Human: Izzo, N. J et al. (2014) PMID: 25390692.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-PGRMC1 antibody (ABIN185610)

Izzo, Xu, Zeng, Kirk, Mozzoni, Silky, Rehak, Yurko, Look, Rishton, Safferstein, Cruchaga, Goate, Cahill, Arancio, Mach, Craven, Head, LeVine, Spires-Jones, Catalano: "Alzheimer's therapeutics targeting amyloid beta 1-42 oligomers II: Sigma-2/PGRMC1 receptors mediate Abeta 42 oligomer binding and synaptotoxicity." in: PLoS ONE, Vol. 9, Issue 11, pp. e111899, (2015) (PubMed).

Target Details for PGRMC1

Target: PGRMC1 (Progesterone Receptor Membrane Component 1 (PGRMC1))

Alternative Name: PGRMC1

Background: PGRMC1, MPR, progesterone receptor membrane component 1 , HGNC:16090, HPR6.6, progesterone binding protein

Gene ID: 10857, 53328

NCBI Accession: NP_006658