TSC1 antibody (C-Term)

Catalog No. ABIN185619

This anti-TSC1 antibody is a Goat Polyclonal antibody detecting TSC1 in ELISA, WB and IHC. Suitable for Human.

Quick Overview for TSC1 antibody (C-Term) (ABIN185619)

Target: TSC1 (Tuberous Sclerosis 1 (TSC1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TSC1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-TSC1 Antibody

Binding Specificity: C-Term

Purpose: Hamartin / TSC1 (isoform 1)

Sequence: QLHIMDYNET HHEH

Specificity: This antibody is expected to recognise isoform 1 (NP_000359.1) only.

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QLHIMDYNETHHEH, from the C Terminus of the protein sequence according to NP_000359.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Uterus and Liver. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 150 kDa band observed in Human Brain lysates (calculated MW of 130 kDa according to NP_000359.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miloloza et al Hum Mol Genet. 2000

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TSC1

Target: TSC1 (Tuberous Sclerosis 1 (TSC1))

Alternative Name: TSC1

Background: TSC1, hamartin, tuberous sclerosis 1 , HGNC:12362, KIAA0243, LAM, MGC86987, TSC, tuberous sclerosis 1 protein, tumor suppressor

Gene ID: 7248, 64930

NCBI Accession: NP_000359

Pathways: RTK Signaling, AMPK Signaling, Regulation of Cell Size, Tube Formation