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Amphiphysin antibody (Internal Region)

Verified AMPH Reactivity: Human, Mouse WB, ELISA, IHC Host: Goat Polyclonal unconjugated
Catalog No. ABIN185628
  • Target See all Amphiphysin (AMPH) Antibodies
    Amphiphysin (AMPH)
    Binding Specificity
    • 15
    • 7
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 45
    • 29
    • 18
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Mouse
    Host
    • 56
    • 6
    • 1
    Goat
    Clonality
    • 59
    • 4
    Polyclonal
    Conjugate
    • 37
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Amphiphysin antibody is un-conjugated
    Application
    • 39
    • 21
    • 13
    • 13
    • 11
    • 8
    • 8
    • 6
    • 3
    • 3
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Purpose
    Amphiphysin / AMPH
    Sequence
    KRKDESRISK AE
    Specificity
    This antibody is expected to recognise both reported isoforms (NP_001626.1 and NP_647477.1).
    Cross-Reactivity
    Dog, Human, Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-KRKDESRISKAE, from the internal region of the protein sequence according to NP_001626.1, NP_647477.1.
    Isotype
    IgG
    Top Product
    Discover our top product AMPH Primary Antibody
  • Application Notes

    Immunohistochemistry: In paraffin embedded Human Cerebellum shows nuclear and fibrous staning. Recommended concentration, 2-4 μg/mL.

    Western Blot: Approx 125 kDa band observed in mouse brain lysates (calculated MW of 76.3 kDa according to NP_001626.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Wigge et al, Mol Biol Cell. 1997

    Peptide ELISA: antibody detection limit dilution 1:16000.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    Amphiphysin (AMPH)
    Alternative Name
    AMPH (AMPH Products)
    Synonyms
    Amp antibody, CG8604 antibody, DAMP antibody, Damp antibody, Dmel\\CG8604 antibody, amph antibody, dAmph antibody, damph antibody, AMPH antibody, Amph antibody, GB16263 antibody, AMPH1 antibody, Amph1 antibody, wu:fq25h04 antibody, zgc:73193 antibody, amphiphysin antibody, Amphiphysin antibody, amphiphysin L homeolog antibody, AMPH antibody, Amph antibody, amph antibody, LOC409851 antibody, amph.L antibody, LOC100343468 antibody
    Background
    AMPH, amphiphysin, amphiphysin (Stiff-Man syndrome with breast cancer 128 kDa autoantigen) , HGNC:471, AMPH1
    Gene ID
    273, 218038
    NCBI Accession
    NP_001626, NP_647477
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