NET1 antibody (Internal Region)

Catalog No. ABIN185644

This Goat Polyclonal antibody specifically detects NET1 in ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for NET1 antibody (Internal Region) (ABIN185644)

Target: NET1 (Neuroepithelial Cell Transforming 1 (NET1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NET1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-NET1 Antibody

Binding Specificity: Internal Region

Purpose: NET1 / ARHGEF8 (Internal)

Sequence: DKLEYLDEKQ RDPR

Specificity: This antibody is expected to recognize both reported isoforms (NP_001040625.1 and NP_005854.2).

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DKLEYLDEKQRDPR, from the internal region of the protein sequence according to NP_005854.2, NP_001040625.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Breast shows cytoplasm staining of myoepithelial cells of ducts. Recommended concentration: 2.5 μg/mL.

Western Blot: Preliminary experiments gave an approx 80-90 kDa double band in Human Placenta lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NET1

Target: NET1 (Neuroepithelial Cell Transforming 1 (NET1))

Alternative Name: NET1

Background: NET1, ARHGEF8, neuroepithelial cell transforming gene 1, NET1A, Rho guanine nucleotide exchange factor (GEF) 8, guanine nucleotide regulatory protein (oncogene), neuroepithelioma transforming gene 1, p65 Net1 proto-oncogene protein, small GTP-binding prot

Gene ID: 10276, 56349, 307098

NCBI Accession: NP_005854, NP_001040625

Pathways: Neurotrophin Signaling Pathway