Catalase antibody (Internal Region)

Catalog No. ABIN185651

The Goat Polyclonal anti-Catalase antibody has been validated for WB, IHC and ELISA. It is suitable to detect Catalase in samples from Human and Mouse.

Quick Overview for Catalase antibody (Internal Region) (ABIN185651)

Target: Catalase (CAT)

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Catalase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-Catalase Antibody

Binding Specificity: Internal Region

Purpose: Catalase / CAT

Sequence: DKYNAEKPKN AIHT

Cross-Reactivity: Dog, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DKYNAEKPKNAIHT, from the internal region of the protein sequence according to NP_001743.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 4-6 μg/mL.

Western Blot: Approx 60 kDa band observed in Human and Mouse Kidney and Liver lysates (calculated MW of 59.8 kDa according to Human NP_001743.1 and Mouse NP_033934.2 ). Recommended concentration: 0.003-0.01 μg/mL. Primary incubation 1 hour at room temperatu

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Catalase

Target: Catalase (CAT)

Alternative Name: CAT

Background: CAT, catalase , HGNC:1516, MGC138422, MGC138424

Gene ID: 847, 12359

NCBI Accession: NP_001743

Pathways: Cellular Glucan Metabolic Process, Cell RedoxHomeostasis, Photoperiodism