Radixin antibody (Internal Region)

Catalog No. ABIN185665

This Goat Polyclonal antibody specifically detects Radixin in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Radixin antibody (Internal Region) (ABIN185665)

Target: Radixin (RDX)

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Radixin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-Radixin Antibody

Binding Specificity: Internal Region

Purpose: Radixin

Sequence: QARDETKKTQ ND

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QARDETKKTQND, from the internal region of the protein sequence according to NP_002897.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Placenta and Kidney. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 80 kDa band observed in Human Lymph Node, Tonsil and Spleen lysates (calculated MW of 68.6 kDa according to NP_002897.1). The same 80 kDa was also observed in lysates of several Mouse and Rat tissues and in lysates of cell lines NIH3T3

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Radixin

Target: Radixin (RDX)

Alternative Name: RDX

Background: RDX, radixin

Gene ID: 5962, 19684, 315655

NCBI Accession: NP_002897

Pathways: Regulation of Actin Filament Polymerization, Asymmetric Protein Localization, SARS-CoV-2 Protein Interactome