UNC13D antibody (Internal Region)

Catalog No. ABIN185677

This Goat Polyclonal antibody specifically detects UNC13D in WB, ELISA and IHC. It exhibits reactivity toward Human and Mouse.

Quick Overview for UNC13D antibody (Internal Region) (ABIN185677)

Target: UNC13D (Unc-13 Homolog D (UNC13D))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This UNC13D antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-UNC13D Antibody

Binding Specificity: Internal Region

Purpose: Munc13-4 / UNC13D (Internal)

Sequence: ETQKHKKDLH PLFD

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-ETQKHKKDLHPLFD

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Thymus shows cytoplasm staining in select areas. Recommended concentration: 2.5 μg/mL. In paraffin embedded Mouse Lung shows staining of the resperatory epithelium in wildtype but not in the knock-out. Data

Western Blot: Approx. 105 kDa band observed in Human T-lymphocyte and HeLa lysates (calculated MW of 123 kDa according to NP_954712.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for UNC13D

Target: UNC13D (Unc-13 Homolog D (UNC13D))

Alternative Name: UNC13D

Background: UNC13D, Munc13-4, unc-13 homolog D (C. elegans) , FHL3, HLH3, HPLH3, unc-13 homolog D

Gene ID: 201294, 70450, 192177

NCBI Accession: NP_954712

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process