CHRM1 antibody (C-Term)

Catalog No. ABIN185701

This Goat Polyclonal antibody specifically detects CHRM1 in WB and ELISA. It exhibits reactivity toward Mouse.

Quick Overview for CHRM1 antibody (C-Term) (ABIN185701)

Target: CHRM1 (Muscarinic Acetylcholine Receptor M1 (CHRM1))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This CHRM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-CHRM1 Antibody

Binding Specificity: C-Term

Purpose: M1 mAChR / CHRM1

Sequence: DKRRWRKIPK RPGSVH

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DKRRWRKIPKRPGSVH, from the C Terminus of the protein sequence according to NP_000729.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 50 kDa band observed in Mouse Heart lysates (calculated MW of 51.4 kDa according to NP_000729.2). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CHRM1

Target: CHRM1 (Muscarinic Acetylcholine Receptor M1 (CHRM1))

Alternative Name: CHRM1

Background: CHRM1, M1 mAChR, cholinergic receptor, muscarinic 1 , HM1, M1, MGC30125 , muscarinic acetylcholine receptor M1

Gene ID: 1128, 12669, 25229

NCBI Accession: NP_000729

Pathways: Synaptic Membrane