ALOX5AP antibody (Internal Region)

Catalog No. ABIN185717

The Goat Polyclonal anti-ALOX5AP antibody has been validated for WB, ELISA and IHC. It is suitable to detect ALOX5AP in samples from Human.

Quick Overview for ALOX5AP antibody (Internal Region) (ABIN185717)

Target: ALOX5AP (Arachidonate 5-Lipoxygenase-Activating Protein (ALOX5AP))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ALOX5AP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-ALOX5AP Antibody

Binding Specificity: Internal Region

Purpose: FLAP / ALOX5AP (internal)

Sequence: HKVEHESRTQ NGR

Specificity: This antibody is expected to recognize both reported isoforms (NP_001620.2, NP_001191335.1).

Cross-Reactivity: Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HKVEHESRTQNGR, from the internal region of the protein sequence according to NP_001620.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Spleen shows staining of select splenocytes. Paraffin embedded Human Lung and Small Intestine. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 16 kDa band observed in lysates of U937 and of Peripheral Blood Mononucleocytes (calculated MW of 18.2 kDa according to NP_001620.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ALOX5AP

Target: ALOX5AP (Arachidonate 5-Lipoxygenase-Activating Protein (ALOX5AP))

Alternative Name: ALOX5AP

Background: ALOX5AP, FLAP, arachidonate 5-lipoxygenase-activating protein, MK-886-binding protein, five-lipoxygenase activating protein

Gene ID: 241

NCBI Accession: NP_001620, NP_001191335