CCM2 antibody (Internal Region)

Catalog No. ABIN185733

This anti-CCM2 antibody is a Goat Polyclonal antibody detecting CCM2 in WB, IF, ELISA and FACS. Suitable for Human and Rat.

Quick Overview for CCM2 antibody (Internal Region) (ABIN185733)

Target: CCM2 (Cerebral Cavernous Malformation 2 (CCM2))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This CCM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-CCM2 Antibody

Binding Specificity: Internal Region

Purpose: CCM2

Sequence: KGEKSRDKKA HEK

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KGEKSRDKKAHEK, from the internal region of the protein sequence according to NP_001025006.1, NP_113631.1, NP_001161407.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 45-48 kDa band observed in Human Heart lysates and approx.48 kDa in Human Cerebral Cortex lysates and in lysates of cell line KNRK (calculated MW of 48.8 kDa according to Human NP_113631.1 and 49.8 kDa according to Rat NP_001119747.1).

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of KNRK cells. Recommended concentration: 10ug/ml.<

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CCM2

Target: CCM2 (Cerebral Cavernous Malformation 2 (CCM2))

Alternative Name: CCM2

Background: CCM2, cerebral cavernous malformation 2 , C7orf22, MGC4067, MGC4607, MGC74868, PP10187

Gene ID: 83605, 216527

NCBI Accession: NP_001025006, NP_113631, NP_001161407

Pathways: Cell-Cell Junction Organization