COL2A1 antibody (AA 1226-1331)

Catalog No. ABIN1858453

This Rabbit Polyclonal antibody specifically detects COL2A1 in WB, IHC, IP and ICC. It exhibits reactivity toward Rat.

Quick Overview for COL2A1 antibody (AA 1226-1331) (ABIN1858453)

Target: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL2A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-COL2A1 Antibody

Binding Specificity: AA 1226-1331

Purpose: Polyclonal Antibody to Collagen Type II Alpha 1 (COL2a1)

Specificity: The antibody is a rabbit polyclonal antibody raised against COL2a1. It has been selected for its ability to recognize COL2a1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPD194Ra01Recombinant Collagen Type II Alpha 1 (COL2a1)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for COL2A1

Target: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Alternative Name: COL2a1

Background: COL2-A1, COL2A1, SEDC, Chondrocalcin, Collagen Alpha-1(II)Chain, Primary Osteoarthritis,Spondyloepiphyseal Dysplasia,Congenital

Pathways: Sensory Perception of Sound, Growth Factor Binding