F12 antibody (AA 400-615)

Catalog No. ABIN1858750

This Rabbit Polyclonal antibody specifically detects F12 in WB, IHC, IP and ICC. It exhibits reactivity toward Human.

Quick Overview for F12 antibody (AA 400-615) (ABIN1858750)

Target: F12 (Coagulation Factor XII (F12))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This F12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-F12 Antibody

Binding Specificity: AA 400-615

Purpose: Polyclonal Antibody to Coagulation Factor XII (F12)

Specificity: The antibody is a rabbit polyclonal antibody raised against F12. It has been selected for its ability to recognize F12 in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Coagulation Factor XII (F12) corresdonding to Ser400~Ser615 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-3 μg/mL,Immunohistochemistry: 5-30 μg/mL,Immunocytochemistry: 5-30 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.24 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for F12

Target: F12 (Coagulation Factor XII (F12))

Alternative Name: Coagulation Factor XII

Background: FXII, HAF, Contact Factor, Hageman Factor

Pathways: Complement System