GCLC antibody (AA 435-636)

Catalog No. ABIN1858972

This Rabbit Polyclonal antibody specifically detects GCLC in WB, IHC, IP and ICC. It exhibits reactivity toward Rat.

Quick Overview for GCLC antibody (AA 435-636) (ABIN1858972)

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCLC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-GCLC Antibody

Binding Specificity: AA 435-636

Purpose: Polyclonal Antibody to Glutamate Cysteine Ligase, Catalytic (GCLC)

Specificity: The antibody is a rabbit polyclonal antibody raised against GCLC. It has been selected for its ability to recognize GCLC in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPD759Ra01Recombinant Glutamate Cysteine Ligase, Catalytic (GCLC)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GCLC

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Alternative Name: GCLC

Background: GCS, GLCL, GLCLC, Gamma-glutamylcysteine synthetase, Gamma-ECS

Pathways: Cell RedoxHomeostasis