PSIP1 antibody (AA 311-469)
Quick Overview for PSIP1 antibody (AA 311-469) (ABIN1859640)
Target
See all PSIP1 AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 311-469
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Purpose
- Polyclonal Antibody to Lens Epithelium Derived Growth Factor (LEDGF)
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Specificity
- The antibody is a rabbit polyclonal antibody raised against LEDGF. It has been selected for its ability to recognize LEDGF in immunohistochemical staining and western blotting.
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Cross-Reactivity
- Human, Rat
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Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
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Immunogen
- RPG671Mu01Recombinant Lens Epithelium Derived Growth Factor (LEDGF)
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Isotype
- IgG
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Application Notes
- Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.
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Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 500 μg/mL
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Buffer
- PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.
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Preservative
- Dithiothreitol (DTT), ProClin
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Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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Handling Advice
- Avoid repeated freeze-thaw cycles.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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Expiry Date
- 12 months
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- PSIP1 (PC4 and SFRS1 Interacting Protein 1 (PSIP1))
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Alternative Name
- LEDGF
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Background
- PSIP1, p75, p52, DFS70, PAIP, PSIP2, PC4 And SFRS1 Interacting Protein 1, CLL-associated antigen KW-7, Dense fine speckles 70 kDa protein, Transcriptional coactivator p75/p52
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Pathways
- Chromatin Binding, Ribonucleoprotein Complex Subunit Organization
Target
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