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NEXN antibody (AA 409-605)

NEXN Reactivity: Human WB, ICC, ELISA, IHC (fro), IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1859994
  • Target See all NEXN Antibodies
    NEXN (Nexilin (NEXN))
    Binding Specificity
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 409-605
    Reactivity
    • 7
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 9
    Rabbit
    Clonality
    • 9
    Polyclonal
    Conjugate
    • 9
    This NEXN antibody is un-conjugated
    Application
    • 7
    • 3
    • 2
    • 2
    • 2
    • 2
    Western Blotting (WB), Immunocytochemistry (ICC), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Sequence
    MGHHHHHHSG SEF- EQ LRQEMGEEEE ENETFGLSRE YEELIKLKRS GSIQAKNLKS KFEKIGQLSE KEIQKKIEEE RARRRAIDLE IKEREAENFH EEDDVDVRPA RKSEAPFTHK VNMKARFEQM AKAREEEEQR RIEEQKLLRM QFEQREIDAA LQKKREEEEE EEGSIMNGST AEDEEQTRSG APWFKKPLKN TSVVDSEPVR FTVKV
    Specificity
    It has been selected for its ability to recognize NEXN in immunohistochemical staining and Western blotting.
    Purification
    Affinity Chromatography
    Immunogen
    Recombinant NEXN expressed in E.coli.
    The antibody is a rabbit polyclonal antibody raised against NEXN.
    Isotype
    IgG
    Top Product
    Discover our top product NEXN Primary Antibody
  • Application Notes
    Western blotting: 1:100-400
    Immunocytochemistry in formalin fixed cells: 1:100-500
    Immunohistochemistry in formalin fixed frozen section: 1:100-500
    Immunohistochemistry in paraffin section: 1:50-200
    Enzyme-linked Immunosorbent Assay: 1:100-200
    Optimal working dilutions must be determined by end user.
    Comment

    Content: The quality control contains recombinant NEXN (Glu409~Val605) disposed in loading buffer.
    Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
    Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
    Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Avoid repeated freeze/thaw cycles
    Storage
    4 °C
    Storage Comment
    Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
    Expiry Date
    12 months
  • Target
    NEXN (Nexilin (NEXN))
    Alternative Name
    Nexilin (NEXN Products)
    Synonyms
    1110046H09Rik antibody, AA553326 antibody, NELIN antibody, CMH20 antibody, nexilin antibody, nexilin F-actin binding protein antibody, nexilin F-actin binding protein S homeolog antibody, nexilin (F actin binding protein) antibody, LOC100422793 antibody, nexn antibody, Nexn antibody, nexn.S antibody, NEXN antibody
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