NME1 antibody (AA 1-152)

Catalog No. ABIN1860003

The Rabbit Polyclonal anti-NME1 antibody (ABIN1860003) specifically detects NME1 in WB, IHC, ICC and IP. The antibody is reactive with Human samples.

Quick Overview for NME1 antibody (AA 1-152) (ABIN1860003)

Target: NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NME1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-NME1 Antibody

Binding Specificity: AA 1-152

Purpose: Polyclonal Antibody to Non Metastatic Cells 1, Protein NM23A Expressed In (NME1)

Specificity: The antibody is a rabbit polyclonal antibody raised against NME1. It has been selected for its ability to recognize NME1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: NME1 (Met1-Glu152)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NME1

Target: NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1))

Alternative Name: NME1

Background: AWD, GAAD, NDPKA, NM23-H1, Nucleoside diphosphate kinase A, Granzyme A-activated DNase, Metastasis inhibition factor nm23, Tumor metastatic process-associated protein

Pathways: Apoptosis, Nucleotide Phosphorylation, Carbohydrate Homeostasis, Ribonucleoside Biosynthetic Process