PDIA3 antibody (AA 162-416)

Catalog No. ABIN1860170

The Rabbit Polyclonal anti-PDIA3 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect PDIA3 in samples from Human.

Quick Overview for PDIA3 antibody (AA 162-416) (ABIN1860170)

Target: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDIA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-PDIA3 Antibody

Binding Specificity: AA 162-416

Purpose: Polyclonal Antibody to Endoplasmic Reticulum Resident Protein 57 (ERp57)

Specificity: The antibody is a rabbit polyclonal antibody raised against ERp57. It has been selected for its ability to recognize ERp57 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Pig, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPB497Hu01Recombinant Endoplasmic Reticulum Resident Protein 57 (ERp57)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.45 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PDIA3

Target: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Alternative Name: ERp57

Background: P58, ERp61, PDIA3, ERp60, GRP57, PI-PLC, GRP58, Glucose Regulated Protein,58 kDa, Protein Disulfide Isomerase-Associated 3, Endoplasmic reticulum resident protein 57

Pathways: Maintenance of Protein Location, Protein targeting to Nucleus, Cell RedoxHomeostasis