PDPK1 antibody (AA 172-399)

Catalog No. ABIN1860172

This Rabbit Polyclonal antibody specifically detects PDPK1 in WB, IHC, ICC and IP. It exhibits reactivity toward Mouse.

Quick Overview for PDPK1 antibody (AA 172-399) (ABIN1860172)

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDPK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-PDPK1 Antibody

Binding Specificity: AA 172-399

Purpose: Polyclonal Antibody to Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1)

Specificity: The antibody is a rabbit polyclonal antibody raised against PDK1. It has been selected for its ability to recognize PDK1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPC718Mu01Recombinant Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mLImmunohistochemistry: 5-20 μg/mLImmunocytochemistry: 5-20 μg/mLOptimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: ProClin, Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PDPK1

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Alternative Name: PDK1

Background: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial

Pathways: PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals