PRAME antibody (AA 416-502)

Catalog No. ABIN1860297

This anti-PRAME antibody is a Rabbit Polyclonal antibody detecting PRAME in WB, IHC, IP and ICC. Suitable for Human.

Quick Overview for PRAME antibody (AA 416-502) (ABIN1860297)

Target: PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRAME antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-PRAME Antibody

Binding Specificity: AA 416-502

Purpose: Polyclonal Antibody to Preferentially Expressed Antigen In Melanoma (PRAME)

Specificity: The antibody is a rabbit polyclonal antibody raised against PRAME. It has been selected for its ability to recognize PRAME in immunohistochemical staining and western blotting.

Cross-Reactivity: Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPH323Hu02Recombinant Preferentially Expressed Antigen In Melanoma (PRAME)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PRAME

Target: PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))

Alternative Name: PRAME

Background: MAPE, OIP4, CT130, Cancer/Testis Antigen 130, Opa-interacting protein 4, Melanoma antigen preferentially expressed in tumors

Pathways: Retinoic Acid Receptor Signaling Pathway, Nuclear Hormone Receptor Binding