Perforin 1 antibody (AA 40-355)

Catalog No. ABIN1860312

This anti-Perforin 1 antibody is a Rabbit Polyclonal antibody detecting Perforin 1 in WB, IHC, IP and ICC. Suitable for Mouse.

Quick Overview for Perforin 1 antibody (AA 40-355) (ABIN1860312)

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Perforin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Perforin 1 Antibody

Binding Specificity: AA 40-355

Purpose: Polyclonal Antibody to Perforin 1 (PRF1)

Specificity: The antibody is a rabbit polyclonal antibody raised against PRF1. It has been selected for its ability to recognize PRF1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Perforin 1 (PRF1) corresdonding to Val40~Lys355 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.23 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: ProClin, Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Perforin 1

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Alternative Name: PRF1

Background: FLH2, HPLH2, P1, PFP, Pore Forming Protein, Cytolysin, Lymphocyte pore-forming protein

Pathways: Apoptosis, Caspase Cascade in Apoptosis