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Pleiotrophin antibody (AA 33-168)

This anti-Pleiotrophin antibody is a Rabbit Polyclonal antibody detecting Pleiotrophin in WB, IHC, IP and ICC. Suitable for Rat.
Catalog No. ABIN1860363

Quick Overview for Pleiotrophin antibody (AA 33-168) (ABIN1860363)

Target

See all Pleiotrophin (PTN) Antibodies
Pleiotrophin (PTN)

Reactivity

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Rat

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This Pleiotrophin antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
  • Binding Specificity

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    AA 33-168

    Purpose

    Polyclonal Antibody to Pleiotrophin (PTN)

    Sequence

    MGHHHHHHSG SEF- GKKEKPEK KVKKSDCGEW QWSVCVPTSG DCGLGTREGT RTGAECKQTM KTQRCKIPCN WKKQFGAECK YQFQAWGECD LNTALKTRTG SLKRALHNAD CQKTVTISKP CGKLTKPKPQ AESKKKKKEG KKQEKMLD

    Specificity

    The antibody is a rabbit polyclonal antibody raised against PTN. It has been selected for its ability to recognize PTN in immunohistochemical staining and western blotting.

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogen

    Recombinant PTN expressed in E.coli.
    The antibody is a rabbit polyclonal antibody raised against PTN.

    Isotype

    IgG
  • Application Notes

    Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    500 μg/mL

    Buffer

    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

    Handling Advice

    Avoid repeated freeze/thaw cycles

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Expiry Date

    12 months
  • Target

    Pleiotrophin (PTN)

    Alternative Name

    Pleiotrophin

    Background

    HARP, HBGF8, HBNF, NEGF1, Heparin Binding Growth Factor 8, Neurite Growth-Promoting Factor 1, Heparin Affin Regulatory Peptide, Heparin Binding Growth Associated Molecule

    Pathways

    RTK Signaling
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