KIT Ligand antibody (AA 24-247) (FITC)

Catalog No. ABIN1860499

This Rabbit Polyclonal antibody specifically detects KIT Ligand in WB, IHC, ICC and IF. It exhibits reactivity toward Mouse.

Quick Overview for KIT Ligand antibody (AA 24-247) (FITC) (ABIN1860499)

Target: KIT Ligand (KITLG)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIT Ligand antibody is conjugated to FITC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-KIT Ligand Antibody (FITC)

Binding Specificity: AA 24-247

Purpose: FITC-Linked Polyclonal Antibody to Stem Cell Factor (SCF)

Sequence: MGHHHHHHSG S-KTKEICG NPVTDNVKDI TKLVANLPND YMITLNYVAG MDVLPSHCWL RDMVIQLSLS LTTLLDKFSN ISEGLSNYSI IDKLGKIVDD LVLCMEENAP KNIKESPKRP ETRSFTPEEF FSIFNRSIDA FKDFMVASDT SDCVLSSTLG PEKDSRVSVT KPFMLPPVAA SSLRNDSSSS NRKAAKAPED SGLQWTAMAL PALISLVIGF AFGALYWKKK QSSLTRA

Specificity: The antibody is a rabbit polyclonal antibody raised against SCF. It has been selected for its ability to recognize SCF in immunohistochemical staining and western blotting.

Cross-Reactivity: Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant SCF expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against SCF conjugated to fitc.

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for KIT Ligand

Target: KIT Ligand (KITLG)

Alternative Name: Stem Cell Factor

Background: KITLG, MGF, SF, KL1, Kitl, FPH2, Kit-Ligand, Steel Factor, Mast Cell Growth Factor, Steel factor, Familial Progressive Hyperpigmentation 2

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway